Insulator-based dielectrophoresis with β-galactosidase in nanostructured devices

摘要: Insulator-based dielectrophoresis (iDEP) has been explored as a powerful analytical technique in recent years. Unlike with larger entities such cells, bacteria or organelles, the mechanism of iDEP transport proteins remains little explored. In this work, we extended pool investigated nanostructured devices β-galactosidase. Our work indicates that β-galactosidase shows concentration due to negative DEP which compare response immunoglobulin G (IgG) encapsulated micelles also showing DEP. Experimental observations are further compared numerical simulations elucidate influence electrokinetic and magnitude mobility. Numerical suggest mobility calculated using classical model underestimates actual contribution on experimentally monitored effect proteins. Moreover, observed unique voltage dependent attribute an additional factor influencing protein at nanoconstrictions, namely ion polarization. aids understanding factors is required for future development preconcentration separation methods based iDEP.