THE RED/WHITE COLONY COLOR ASSAY IN THE YEAST SACCHAROMYCES CEREVISIAE : EPISTATIC GROWTH ADVANTAGE OF WHITE ADE8-18, ADE2 CELLS OVER RED ADE2 CELLS

摘要: In the yeast Saccharomyces cerevisiae ade2, and/or ade1, mutation in adenine biosynthetic pathway leads to accumulation of a cell-limited red pigment, while epistatic mutations same pathway, i.e. ade8, preclude this phenomenon, resulting normal white colonies. The shift color from (or vice versa) with combination appropriate wild-type and mutant alleles adenine-pathway genes has been widely utilized as non-selective phenotype visualise quantify occurrence various genetic events such recombination, conversion aneuploidy. It provided an invaluable tool for study gene dosage plasmid stability. competition experiments between disrupted ade8-18 transformants carrying either functional or non-functional episomal ADE8 gene, we verified that ade8 ade2 cells show remarkable selective advantage over cells, important implications on use assay monitoring events. pigment is likely be cause impaired growth these cells.