Detecting carcinogens with the yeast DEL assay.
作者： Richard J. Brennan , Robert H. Schiestl
DOI: 10.1385/1-59259-761-0:111
关键词: Auxotrophy 、 Genotoxicity 、 Carcinogen 、 Yeast 、 Gene 、 Histidine 、 Biochemistry 、 Intrachromosomal recombination 、 Chemistry 、 Reversion
摘要: The yeast DEL assay is a simple, rapid method for measuring the frequency of reversion disrupted his3 gene by homologous intrachromosomal recombination. Reversion to histidine prototrophy results in deletion (DEL) disrupting sequence. has been used study effects various DNA-damaging treatments on deletion-recombination and shown have high level sensitivity specificity toward carcinogens, many which are poorly detected bacterial mutagenicity other short-term genotoxicity assays. therefore useful addition arsenal predictive tests carcinogenicity. This chapter provides an in-depth description materials methods from user's prospective should allow be successfully deployed any laboratory with basic microbiological capability minimal user training.
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