HNO induces DNA deletions in the yeast S. cerevisiae.

摘要: Abstract HNO is genotoxic but its mechanism not well understood. There are many possible mechanisms by which can attack DNA. Since electrophilic, it may react with exocyclic amine groups on DNA bases and through a series of subsequent reactions form deaminated product. Alternatively, induce radical chemistry O2-dependent (or possibly O2-independent) chemistry. In cell free systems, experiments have shown that does DNA, resulting in base oxidation strand cleavage. this study, we used whole-cell system the yeast Saccharomyces cerevisiae to study induced damage Angeli's salt as donor. The DEL assay provided measure intrachromosomal recombination leading deletions. We also examined interchromosomal genomic rearrangements canavanine (CAN) induction forward point mutations. was potent inducer deletions negative for This suggests causes breaks rather than damage. Genotoxicity observed under aerobic anaerobic conditions NAC protected against conditions, probably radicals generated independent oxygen.