The aromatic amine carcinogens o-toluidine and o-anisidine induce free radicals and intrachromosomal recombination in Saccharomyces cerevisiae

摘要: Aniline-based aromatic amine carcinogens are poorly detected in short-term mutagenicity assays such as the Salmonella reverse mutation (Ames) assay. More information on mechanism of toxicity Salmonella-negative is needed. Aniline and o-toluidine negative Ames assay, but induce deletions (DEL) due to intrachromosomal recombination Saccharomyces cerevisiae with an apparent threshold. We show here that DEL assay also detects genotoxic activity another carcinogen, o-anisidine, which distinguishes between o-anisidine its non-carcinogenic structural analog 2,4-dimethoxyaniline. have investigated whether ability detect distinguish carcinogen/non-carcinogen pair linked rises intracellular free radical species following exposure carcinogens. Toxicity induced by all three compounds was reduced presence scavenger antioxidant N-acetyl cysteine, cysteine. All oxidation radical-sensitive reporter compound dichlorofluorescin diacetate. Superoxide dismutase-deficient strains, however, were hypersensitive cytotoxicity not non-carcinogen 2,4-dimethoxyaniline, indicating a different potential for generating superoxide analog. The results indicate yeast useful tool investigating