Universal neonatal cytomegalovirus screening using saliva - report of clinical experience.

摘要: Abstract Objectives To analyze the results of a neonatal universal screen for congenital cytomegalovirus (CMV) using saliva real-time polymerase chain reaction (rt-PCR). Study design During one year (15/5/2011–15/5/2012), was collected from 9845 infants (97% 10,137 newborns). Viral DNA extracted by Magna-Pure LC (Roche) and tested presence CMV IE gB genes. Urine culture positive confirmation. For all with maternal data were head ultrasound, blood count, liver enzymes, retinal examination auditory brainstem response testing performed. Parents notified in advance had option to avoid screening. The ethical committee approved retrospective analysis data. Results Fifty six (0.57%) assay. Of these, 47 confirmed urine rt-PCR culture, another sero-conversion documented during pregnancy (48 infants). Twenty-eight mothers (28/47, 60%) primary infection pregnancy, 14 (30%) non-primary infection, no serological obtained five (10%). Four (8.5%), two prenatal diagnosis normal fetal brain imaging born sero-positive before exhibited symptoms related offered antivirals. Hearing impairment diagnosed (late onset HI case). Conclusions Saliva assay is feasible effective means screening that can detect affected who might benefit treatment follow-up. long-term clinical significance its cost effectiveness are yet be determined.