Identification of Alternaria brassicicola genes expressed in planta during pathogenesis of Arabidopsis thaliana.

摘要: Alternaria brassicicola is a necrotrophic fungal pathogen that causes black spot disease on cruciferous plants including economically important Brassica species. The purpose of this study was to identify genes expressed during infection Arabidopsis. In order candidate involved in pathogenicity, we employed suppression subtractive hybridization (SSH) between RNA isolated from A. spores incubated water and the leaf surface Arabidopsis ecotype Landsberg. Two populations cDNA were created total extracted after 24h when approximately 80% had germinated either or water. Following SSH, expression clones examined using dot-blot macro-arrays virtual Northern blots. 47 differentially infected leaves spore germination selected for sequencing. Seventy-seven percent (36) cDNAs significant homology sequences databases examined, available genomes, while 13% (11) no databases. All 36 matches with origin, 11 did not have hits examined. Five plant but according These five predicted encode cyanide hydratase, arsenic ATPase, formate dehydrogenase, major allergen, one unknown. RT-PCR used examine these oleraceae var. capitata (cabbage), vitro growth nutrient rich media, thaliana. Four are medium, unknown gene P3F2 only infection. results provide first insight into species may be pathogenesis.