clap1, a gene encoding a copper-transporting ATPase involved in the process of infection by the phytopathogenic fungus Colletotrichum lindemuthianum.

摘要: A screen for insertional mutants of Colletrichum lindemuthianum, the causative agent common bean anthracnose, led to identification a non-pathogenic, lightly colored transformant. This mutant is unable induce disease symptoms on intact or wounded primary leaves seedlings and plantlets Phaseolus vulgaris. In vitro, it exhibits normal vegetative growth, sporulation conidial germination, but cultures remain beige instead becoming black. Microscopic examination revealed that this forms fewer appressoria than wild-type strain, these are misshapen poorly melanized. Molecular analyses indicated mutagenic plasmid had targeted clap1, gene encoding putative copper-transporting ATPase sharing 35% identity with human Menkes Wilson proteins product CCC2 Saccharomyces cerevisiae. Complementation non-pathogenic allele clap1 restored both pathogenicity pigmentation. Conversely, replacement disrupted gave rise transformants. Compared extracts from were found have very low levels phenol oxidase activity. These observations suggest may be involved in C. lindemuthianum strains because its role delivering copper secreted cuproenzymes, such as oxidases mediate polymerization 1,8-dihydroxynaphthalene melanin.