Modulation of phospholipase C pathway and level of Gq alpha/G11 alpha in rat myometrium during gestation.
作者： S. Lajat , Z. Tanfin , G. Guillon , S. Harbon
DOI: 10.1152/AJPCELL.1996.271.3.C895
关键词: Endocrinology 、 Myometrium 、 Internal medicine 、 Phosphatidylinositol 、 Inositol 、 Chemistry 、 G protein 、 Phospholipase C 、 Muscarinic acetylcholine receptor 、 Alpha (ethology) 、 Pertussis toxin
摘要: The regulation of the receptor-G protein-phospholipase C (PLC) cascade was investigated in rat myometrium at midgestation (day 12) and term 21) comparatively to estrogen-treated tissue 0). Carbachol-mediated generation [3H]inositol phosphates insensitive pertussis toxin enhanced days 12 21 two- threefold, respectively, with no alteration muscarinic sites (M3 subtype). A similar increase could be detected production inositol 1,4,5-trisphosphate, indicating stimulation a PLC degrading phosphatidylinositol 4,5-bisphosphate. AlF4- also activation during gestation, suggesting pregnancy-related regulations that bypass receptor activation. Immunoreactive G proteins, Gq alpha G11 alpha, PLC-beta 3 were all myometrial preparations. amount day 0 myometrium, although decreasing by 75% midgestation. Of significance increased (3- 2-fold, respectively) which coincided phosphoinositide breakdown. upregulation may contribute activity pregnancy term.
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