Tissue microarray use for immunohistochemical study of ameloblastoma.

摘要: Background Ameloblastoma is a locally aggressive odontogenic tumor with high rates of recurrence. To better understand the molecular basis ameloblastoma, tissue microarray (TMA) may represent useful tool. However, despite TMA has been considered high-throughput technique for different human neoplasms, it remains to be validated in ameloblastoma context. Therefore, objective this study was validate immunohistochemical determining its most appropriate design. Methods Forty cases were manually distributed two blocks assembled triplicate containing 1.0- and 2.0-mm cores (20 each). Immunohistochemistry cytokeratins 14 19, Bcl-2 Ki-67 performed, semiquantitative analysis performed. Results obtained sections compared their corresponding conventional whole-section slides (CWSS). Results Kappa statistical test demonstrated that both assessed as duplicate or significantly correlated CWSS, higher levels using Ki67 (k = 0.98, 0.97, 0.88, 0.87) CK19 0.62, 0.58, 0.85, 0.85). There no significant difference between cores, values. 1.0-mm showed index core loss (33.74% vs. 4.99%). Conclusion Using manual arrayer, arranged valid method satisfactory agreement cylinders CWSS.