Validation of a tissue microarray to study differential protein expression in inflammatory and non-inflammatory breast cancer
作者： G.G. Van den Eynden , I. Van der Auwera , S. Van Laere , C.G. Colpaert , P. van Dam
DOI: 10.1023/B:BREA.0000021028.33926.A8
关键词:
摘要: Summary Aims. Inflammatory breast cancer (IBC) is an aggressive subtype of with poor prognosis. The mechanisms responsible for the clinical evolution are incompletely understood. We constructed a tissue microarray (TMA) and validated its use in translational IBC research. Differential expression proteins that might play role causing phenotype was studied. Methods results. A TMA containing 34 41 non-stage matched non-IBC tumours constructed. Five core biopsies were taken each three cores tumour. using approaches: (1) excellent concordance between immunohistochemical results initial pathological examination obtained ER, PR HER2/neu ( κ> 0.74); (2) known differential four bio-markers (ER, PR, p53 HER2/neu) confirmed p 0.75). Furthermore, overexpression E-Cadherin RhoC GTPase < 0.05) confirmed. did not find pattern carbonic anhydrase IX (CA IX) EGFR. Conclusions. Using different approaches, we have our studying protein non-IBC. confirm IBC. lack CA EGFR suggest pathways equally utilised both types cancer.
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