Amphetamine: evaluation of d- and l-isomers as releasing agents and uptake inhibitors for 3H-dopamine and 3H-norepinephrine in slices of rat neostriatum and cerebral cortex.

摘要: Release of 3H-doapamine or 3H-norepinephrine and inhibition accumulation 3H-dopamine by d- l-amphetamine were studied in slices rat neostriatum cerebral cortex. The two stereoisomers amphetamine equally potent as inhibitors the cortex, whereas d-amphetamine was approximately 3-fold more than neostriatum. A similar relationship observed between release experiments. Some spontaneous efflux from tissue evident absence added drug When cocaine (2 X 10(-5) M), a known inhibitor biogenic amine uptake, to medium, there very little increment neostriatal slices, but blocked caused d-amphetamine. This showed that apparent releasing action not due blockade reuptake spontaneously released material itself must be taken up evoke action. Experiments designed compare directly uptake inhibiting actions In greater over wide concentration range, while essentially identical magnitude for 3H-dopamine. We conclude can act both block 3H-norepinephrine. neostriatum, on other hand, is (which stronger cortex), "uptake" questionable significance appears result previously accumulated