MHC interaction and T cell recognition of carbohydrates and glycopeptides.

摘要: The T cell independence of complex polysaccharide Ag has suggested the possibility that carbohydrates may be incapable recognition because a failure to interact with MHC restriction elements and/or MHC/carbohydrate complexes and recognized by Ag-specific TCR. We have used two approaches obtain information about carbohydrate. First, we determined capacity series oligosaccharides glycolipids bind murine class II molecule, IAd. No significant binding was observed 26 compounds tested, but limitation these studies there relatively limited collection synthetic carbohydrate glycolipid structures complexity available for analysis. second approach involved study effect glycosylation known peptide epitope (OVA 323-339) on recognition. Three patterns effects were observed: 1) no either or This pattern when located at residues removed from core MHC-binding region. When within regions, 2) destroyed both recognition; 3) did not ablate In this latter instance, evidence indicate moiety an important part antigenic determinant cells.