Functional Reconstitution of a Prokaryotic K+ Channel
作者： Lise Heginbotham , Ludmila Kolmakova-Partensky , Christopher Miller
关键词: Selectivity 、 Biophysics 、 Ion transporter 、 KcsA potassium channel 、 Potassium channel 、 Flux (metabolism) 、 Chemistry 、 Biochemistry 、 Permeation 、 Kinetics 、 Liposome
摘要: SliK, a K+ channel encoded by the Streptomyces KcsA gene, was expressed, purified, and reconstituted in liposomes. A concentrative 86Rb+ flux assay used to assess ion transport properties of SliK. SliK-mediated ionic shows strong selectivity for over Na+ is inhibited micromolar concentrations Ba2+, mirroring basic permeation characteristic eukaryotic channels studied electrophysiological methods. uptake kinetics equilibrium measurements also demonstrate that purified protein fully active.
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