Small cell carcinomas of the lung express the Bcl-2 protein.

摘要: In our laboratory, we observed that a case of small cell carcinoma the lung (SCLC) stained with monoclonal antibody (Dako Corp., Bcl-2-124) against Bcl-2 protein. To determine how common this reaction was, and whether it was specific for SCLC, formalin-fixed, paraffin-embedded tissues from 23 cases SCLC 20 squamous Bcl-2. Fifteen positively oncogene contrast, weak staining in only three carcinomas (chi 2-test; P = 0.001). addition, four lines (H69, H146, H209, WB) were tested by immunohistochemistry flow cytometry expression protein; all positive. these other (H128, H432, H510) lines, Northern blots polyadenylated RNA revealed 6-kb mRNA. Moreover, Western extracts characteristic 26-kd band single line, H82, which has previously been characterized as variant failed to detect Bcl-2-specific mRNA We therefore conclude most express oncoprotein, could play role pathogenesis disease.