Genome-wide Analysis Reveals New Roles for the Activation Domains of the Saccharomyces cerevisiae Heat Shock Transcription Factor (Hsf1) during the Transient Heat Shock Response

摘要: In response to elevated temperatures, cells from many organisms rapidly transcribe a number of mRNAs. Saccharomyces cerevisiae, this protective involves two regulatory systems: the heat shock transcription factor (Hsf1) and Msn2 Msn4 (Msn2/4) factors. Both systems modulate induction specific genes. However, contribution Hsf1, independent Msn2/4, is only beginning emerge. To address question, we constructed an msn2/4 double mutant used microarrays elucidate genome-wide expression program Hsf1. The data showed that 7.6% genome was heat-induced. up-regulated genes belong wide range functional categories, with significant increase in chaperone metabolism We then focused on activation domains Hsf1 profile extended our analysis include msn2/4Δ strains deleted for N-terminal or C-terminal domain Cluster heat-induced revealed domain-specific patterns expression, each cluster also showing distinct preferences categories. Computational promoters induced affected by loss preference positioning topology binding site. This study provides insight into important role both play system effectively its regulon shock.