Method to predict and compare the influence of the particle size on the isocratic peak capacity of high-performance liquid chromatography columns.

摘要: Abstract A kinetic plot based method has been used to experimentally predict the optimal particle size yielding maximal isocratic peak capacity in a given analysis time. Applying columns of three different manufacturers and characterizing them by separating 4-component paraben mixture at 30 °C, it was consistently found that classical 3 3.5 μm particles provide highest for typical separation run times between 30 60 min when operating conventional pressure 400 bar. Even 1000 bar, sub-2 μm only have distinct advantage runs lasting 30 min or less, while 45 min longer again are be preferred. This finding points high-resolution separations could obtained producing can operated elevated pressures.