Metabolism of Chlorotoluenes by Burkholderia sp. Strain PS12 and Toluene Dioxygenase of Pseudomonas putida F1: Evidence for Monooxygenation by Toluene and Chlorobenzene Dioxygenases.
作者： A Lehning , U Fock , R Wittich , K N Timmis , D H Pieper
DOI: 10.1128/AEM.63.5.1974-1979.1997
关键词: Toluene 、 Pseudomonas putida 、 Toluene dioxygenase 、 Chemistry 、 Pseudomonadales 、 Chlorobenzene 、 Chlorobenzoates 、 Stereochemistry 、 Burkholderia 、 Pseudomonadaceae 、 Organic chemistry
摘要: The degradation of toluene by Pseudomonas putida F1 and chlorobenzenes Burkholderia sp. strain PS12 is initiated incorporation dioxygen into the aromatic nucleus to form cis-dihydrodihydroxybenzenes. Toluene-grown cells P. 3-chlorobenzoate-grown were found monooxygenate side chain 2- 3-chlorotoluene corresponding chlorobenzyl alcohols. Further metabolism these products was slow, chlorobenzoates usually observed as end products, whereas 3-chlorobenzoate produced from in metabolized further. Escherichia coli containing dioxygenase genes oxidized alcohols major demonstrating that this enzyme responsible for monooxygenation. Two methyl- chloro-substituted 1,2-dihydroxycyclohexadienes formed minor 3-chlorotoluene, a chloro- methyl-substituted cyclohexadiene only product 4-chlorotoluene. chlorobenzene are first enzymes described efficiently catalyze oxidation 2-chlorotoluene.
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