Three salvianolic acids inhibit 2019-nCoV spike pseudovirus viropexis by binding to both its RBD and receptor ACE2.

摘要: Since December 2019, the new coronavirus [also known as severe acute respiratory syndrome 2 (SARS-CoV-2, 2019-nCoV)]- induced disease, COVID-19, has spread rapidly worldwide. Studies have reported that traditional Chinese medicine Salvia miltiorrhiza possesses remarkable antiviral properties; however, anti-coronaviral activity of its main components, salvianolic acid A (SAA), B (SAB), and C (SAC) is still debated. In this study, we used Cell Counting Kit-8 staining flow cytometry to evaluate toxicity SAA, SAB, SAC on ACE2 (angiotensin-converting enzyme 2) high-expressing HEK293T cells (ACE2h cells). We found SAB had a minor effect viability ACE2h at concentrations below 100 μM. further evaluated binding capacity spike protein 2019-nCoV using molecular docking surface plasmon resonance (SPR). They could bind receptor-binding domain (RBD) with constant (KD ) (3.82±0.43) e-6 M, (5.15±0.64)e-7 (2.19±0.14)e-6 M; KD (4.08±0.61)e-7 (2.95±0.78)e-7 M (7.32±0.42)e-7M, respectively. As result, were determined inhibit entry Spike pseudovirus EC50 11.31μM, 6.22 μM, 10.14 μM cells, conclusion, our study revealed three Salvianolic acids can into by RBD protein. This article protected copyright. All rights reserved.