Identification of the major constituents in Xuebijing injection by HPLC-ESI-MS.

摘要: Introduction – Xuebijing injection (XBJ) is a traditional Chinese herbal prescription widely used in the treatment of sepsis. This first report concerning identification XBJ constituents. In addition, to evaluate XBJ's quality, partial least square discrimination analysis (PLS-DA) was performed on chemical fingerprint data. Objective Establish an LC-MS method identify components for purpose quality control. Methodology Compounds were separated by HPLC using C18 column and gradient elution acetonitrile–methanol (60:40, v/v) water–acetic acid (100 : 0.5, 80 min. equipped with diode array detector (DAD) coupled time-of-flight (TOF) tandem mass spectrometry electrospray ionisation (ESI) multi-stage ion-trap (IT-MSn) developed analyse major components. Both positive negative modes employed. Results Twenty-one compounds including amino acids, phenolic flavonoid glycoside, terpene glycoside phthalide identified or tentatively characterised. Their retention times, UV MS spectra compared those authentic literature data. The score plot PLS-DA clearly revealed variations among samples produced different commercial batches. Conclusion analytical highly effective control XBJ.