Determination of total platinum in plasma and plasma ultrafiltrate, from subjects dosed with the platinum-containing N-(2-hydroxypropyl)methacrylamide copolymer AP5280, by use of graphite-furnace Zeeman atomic-absorption spectrometry

摘要: AP5280 is an N-(2-hydroxypropyl)methacrylamide (HPMA) copolymer to which are linked tetrapeptide side chains containing bioactive platinum complexes at their C-terminal sides. We have developed and validated a rapid sensitive analytical assay for the determination of total concentrations in plasma, free origin plasma ultrafiltrate (PUF), subjects dosed with AP5280. The levels were determined by use graphite-furnace atomic-absorption spectrometry (GF-AAS) Zeeman correction after appropriate dilution sample plasma–hydrochloric acid 0.2 mol L–1 (1:5) as diluent. limit quantitation this 0.25 µmol plasma. Linear calibration curves obtained over concentration range 0.25–5.0 L–1. Accuracy was between 87.7% 104.2% precision 15.3% lowest less than 14% all other tested. thus accordance generally accepted criteria methods. Analysis samples from patients receiving demonstrated applicability described assay.