Comparison of Two Approaches for the Metataxonomic Analysis of the Human Milk Microbiome.

摘要: Recent work has demonstrated the existence of large inter-individual and inter-population variability in microbiota human milk from healthy women living across variable geographical socio-cultural settings. However, no studies have evaluated impact that sequencing approaches targeting different 16S rRNA regions may on profiling results. This hampers our ability to make meaningful comparisons studies. In this context, main purpose present study was re-process re-sequence microbiome a set samples (n = 412) collected at diverse international sites (Spain, Sweden, Peru, United States, Ethiopia, Gambia, Ghana Kenya), by region reaching larger depth. Despite some differences between results obtained both were notable (especially regarding alpha beta diversities Proteobacteria representation), indicate revealed relatively consistent configurations studied cohorts. Our data expand upon we previously reported INSPIRE cohort provide, for first time globally populations, evidence DNA processing profiles samples. Overall, corroborate similarities microbial communities cohort, but also detected. Understanding is essential enable Clinical Trial Registration www.clinicaltrials.gov, identifier NCT02670278.