Regulation of actin mRNA levels and translation responds to changes in cell configuration.

摘要: The role of cell configuration in regulating metabolism has been studied, using a system which shape and surface contact can easily be manipulated. suspension anchorage-dependent mouse fibroblasts Methocel results coordinate decrease DNA, RNA, protein synthesis. These processes are restored upon reattachment cells to solid surface. This recovery process two or more components: rapid synthesis requiring only contact, slower restoration nuclear events is dependent extensive spreading (A. Ben-Ze'ev, S.R. Farmer, S. Penman, Cell 21:365-372, 1980). In the present study, we examined 3T3 while culture after attachment tissue dish study configuration-dependent expression specific cytoskeleton genes. line used here shows these responses much dramatically compared with 3T6 previously studied. We demonstrate that whereas total was strongly inhibited suspension, actin preferentially inhibited, decreasing from 12% control 6% suspended cells. occurred apparently at level translation mRNA, since amount mRNA sequences cytoplasm unchanged. Reattachment initiated overall accompanied by dramatic, preferential increase reaching peak values 20 25% 4 6 h later, but then declining 24 h. Translation vitro hybridization cloned cDNA probe revealed induction due increased levels translatable cytoplasm. imply close relationship among cytoarchitecture, cytoskeletal gene, growth control. gene appears regulated both (during suspension) production recovery).