Effect of increased glutamate availability on L-ornithine production in Corynebacterium glutamicum.

摘要: Glutamate availability in the argF-argR-proBDelta strain of Corynebacterium glutamicum was increased by addition glutamate to cell or inactivation phosphoenolpyruvate carboxykinase activity and simultaneous overexpression pyruvate carboxylase assess its effect on Lornithine production. When an Lornithine- producing strain, production L-ornithine not changed. This unexpected result indicated that intracellular concentration supply is a rate-limiting step for L-ornithine-producing C. glutamicum. In contrast, biosynthesis genes (argCJBD) resulted approximately 30% increase production, from 12.73 16.49 mg/g (dry weight). These results implied downstream reactions converting L-ornithine, but glutamate, elevating