Metabolic engineering of Corynebacterium glutamicum for the production of L‐ornithine
作者： Seo Yun Kim , Joungmin Lee , Sang Yup Lee
DOI: 10.1002/BIT.25440
关键词:
摘要: L-ornithine is a non-essential amino acid for various industrial applications in food industry. In this study, Corynebacterium glutamicum ATCC 13032 was metabolically engineered the production of L-ornithine. First, proB and argF genes were deleted to block competitive branch pathway conversion citrulline, respectively. addition, argR gene encoding regulatory repressor L-arginine operon also deleted. The resulting strain produced 230 mg/L from glucose flask culture. This base further by plasmid-based overexpression argCJBD C. 21831, which resulted 7.19 g/L To enrich NADPH pool, carbon flux redirected towards pentose phosphate changing start codons pgi zwf replacing native promoter tkt with strong sod promoter. Fed-batch cultivation final YW06 (pSY223) allowed 51.5 g/L 40 h overall productivity 1.29 g/L/h. results obtained study demonstrate possibility efficiently producing glutamicum. Biotechnol. Bioeng. 2015;112: 416–421. © 2014 Wiley Periodicals, Inc.
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