Identification of hydatidosis-related modules and key regulatory genes.

摘要: Background Echinococcosis caused by larval of Echinococcus is prevalent all over the world. Although clinical experience showed that presence tapeworms could not be found in liver lesions, repeated infection and aggravation lesions still occur host. Here, this study constructed a multifactor-driven disease-related dysfunction network to explore potential molecular pathogenesis mechanism different hosts after E.multilocularis infection. Method First, iTRAQ sequencing was performed on human infected with E.multilocularis. Second, obtained microRNAs(miRNAs) expression profiles humans canine from GEO database. In addition, we also differential analysis, protein interaction enrichment crosstalk analysis obtain genes modules related Pivot used calculate regulatory effects multiple factors module identify non-coding RNAs(ncRNAs) transcription factors(TFs). Finally, screened target miRNAs further its mechanism. Results A total 267 differentially expressed proteins 3,635 were obtained. They participated 16 human-related five canine-related modules, respectively. Both are significantly involved BMP signaling pathway TGF-beta pathway. pivot 1,129 ncRNAs 110 TFs regulated 158 nine modules. Surprisingly, miR-184 plays role pathogenicity regulation targeting one ncRNA humans. Similarly, can cause physiological regulating two canine. Conclusion The results show miRNA-184 regulate pathogenic process through various biological functions pathways. laid solid theoretical foundation for biologists Echinococcosis.