Phosphorylation of nuclear and cytoplasmic pools of ribosomal protein S6 during cell cycle progression

摘要: Of all known ribosomal proteins, the 40S protein S6 is by far most extensively studied. Still, little about some basic aspects of regulation including its cell cycle-related expression and localization. Using a flow cytometric single approach applied to whole cells isolated nuclei, we monitored nucleocytoplasmic total S240/4 phosphorylated during unperturbed cycle progression, providing first evidence for S6-specific spatiotemporal pattern deregulation under conditions hyperactivated mTOR.