Effect of the DGAT1 K232A genotype of dairy cows on the milk metabolome and proteome
作者： Jing Lu , Sjef Boeren , Toon van Hooijdonk , Jacques Vervoort , Kasper Hettinga
关键词: Diglyceride 、 Orotic acid 、 Metabolite 、 Biochemistry 、 Metabolome 、 Biology 、 Triglyceride 、 Metabolomics 、 Sugar 、 Carnitine
摘要: Diglyceride O-acyltransferase 1 (DGAT1) is the enzyme that catalyzes synthesis of triglycerides from diglycerides and acyl-coenzyme A. The DGAT1 K232A polymorphism was previously shown to have a significant influence on bovine milk production characteristics (milk yield, protein content, fat fatty acid composition). mechanism this has, however, not been elucidated. In study, metabolomics (1H-nuclear magnetic resonance) proteomics (laser chromatography-tandem mass spectrometry) were applied determine serum lipid metabolite composition globule membrane proteome samples cows with KK AA genotypes. genotype contained more stomatin, sphingomyelin, choline, carnitine, less citrate, creatine or phosphocreatine, glycerol-phosphocholine, mannose-like sugar, acetyl sugar phosphate, uridine diphosphate (UDP)-related orotic compared genotype. Based these results, we propose differences between genotypes may be related stomatin-sphingomyelin rafts as well structural (cell membrane) in epithelial cells mammary gland. conclusion, our study shows that, addition described changes triglyceride composition, differing differ their metabolome, which help further understanding effect characteristics.
core.ac.uk
sci-hub.se 参考文章(36)
A. Schennink, W. M. Stoop, M. H. P. W. Visker, J. M. L. Heck, H. Bovenhuis, J. J. Van Der Poel, H. J. F. Van Valenberg, J. A. M. Van Arendonk, DGAT1 underlies large genetic variation in milk‐fat composition of dairy cows Animal Genetics. ,vol. 38, pp. 467- 473 ,(2007) , 10.1111/J.1365-2052.2007.01635.X
R Prohaska, D. Thinessempoux, L. Snyers, Colocalization of stomatin (band 7.2b) and actin microfilaments in UAC epithelial cells. European Journal of Cell Biology. ,vol. 73, pp. 281- 285 ,(1997)
Justin J.J. van der Hooft, Velitchka Mihaleva, Ric C.H. de Vos, Raoul J. Bino, Jacques Vervoort, A strategy for fast structural elucidation of metabolites in small volume plant extracts using automated MS-guided LC-MS-SPE-NMR. Magnetic Resonance in Chemistry. ,vol. 49, ,(2011) , 10.1002/MRC.2833
Maria Vinaixa, Miguel Ángel Rodríguez, Anna Rull, Raúl Beltrán, Cinta Bladé, Jesús Brezmes, Nicolau Cañellas, Jorge Joven, Xavier Correig, Metabolomic Assessment of the Effect of Dietary Cholesterol in the Progressive Development of Fatty Liver Disease Journal of Proteome Research. ,vol. 9, pp. 2527- 2538 ,(2010) , 10.1021/PR901203W
Wieland B Huttner, Joshua Zimmerberg, Implications of lipid microdomains for membrane curvature, budding and fission. Current Opinion in Cell Biology. ,vol. 13, pp. 478- 484 ,(2001) , 10.1016/S0955-0674(00)00239-8
Paul J Boersema, Reinout Raijmakers, Simone Lemeer, Shabaz Mohammed, Albert J R Heck, Multiplex peptide stable isotope dimethyl labeling for quantitative proteomics. Nature Protocols. ,vol. 4, pp. 484- 494 ,(2009) , 10.1038/NPROT.2009.21
Christelle Lopez, Marie-Noëlle Madec, Rafael Jimenez-Flores, Lipid rafts in the bovine milk fat globule membrane revealed by the lateral segregation of phospholipids and heterogeneous distribution of glycoproteins Food Chemistry. ,vol. 120, pp. 22- 33 ,(2010) , 10.1016/J.FOODCHEM.2009.09.065
S.R. Cardoso, L.B. Queiroz, V. Alonso Goulart, G.B. Mourão, E. Benedetti, L.R. Goulart, Productive performance of the dairy cattle Girolando breed mediated by the fat-related genes DGAT1 and LEP and their polymorphisms. Research in Veterinary Science. ,vol. 91, ,(2011) , 10.1016/J.RVSC.2011.02.006
Alison Steiber, Janos Kerner, Charles L Hoppel, Carnitine: a nutritional, biosynthetic, and functional perspective Molecular Aspects of Medicine. ,vol. 25, pp. 455- 473 ,(2004) , 10.1016/J.MAM.2004.06.006
Ellen Umlauf, Edina Csaszar, Manuel Moertelmaier, Gerhard J. Schuetz, Robert G. Parton, Rainer Prohaska, Association of Stomatin with Lipid Bodies Journal of Biological Chemistry. ,vol. 279, pp. 23699- 23709 ,(2004) , 10.1074/JBC.M310546200