Simultaneous isolation of major viral proteins in one step.
作者： Hartmut Schetters , Beverly McLeod
DOI: 10.1016/0003-2697(79)90149-0
关键词: Molecular biology 、 Virus 、 Isolation (microbiology) 、 Radioimmunoassay 、 Sodium 、 Biology 、 Electrophoresis 、 RNA 、 Tumor Virus 、 Gel electrophoresis
摘要: Abstract We have developed a rapid and simple technique for the simultaneous isolation of all major viral proteins from RNA tumor viruses. The basis this procedure is analytical sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Using dansylated virus as internal marker it possible to follow migration unlabeled since dansylation does not change mobility labeled (8). method results in approximately 80% recovery starting protein very reproducible. radioimmunoassay no alteration purified detectable.
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