Generation of human T lymphocytes from bone marrow CD34+ cells in vitro.

摘要: Analysis of the events that regulate development red blood cells or granulocytes has led to therapies altering clinical conditions associated with anemia neutropenia. The therapeutic approaches target lymphopenia, such as AIDS, been thwarted by limited techniques for studying T–lymphocyte development. We describe an in vitro system which human bone marrow CD34+ proliferate, acquire expression lymphoid–specific RAG–2 gene and a broad repertoire rearranged T–cell receptor genes, develop ability produce T cell–specific interleukin–2 achieve range immunophenotypes. also become susceptible infection T–lymphotropic strain immunodeficiency virus–1, HIV–1IIIB. This culture induces lymphopoiesis may permit further analysis regulating T–lineage differentiation. It provides preclinical model screening stem cell directed toward AIDS.