Gene Expression Profiling of Listeria monocytogenes Strain F2365 during Growth in Ultrahigh-Temperature-Processed Skim Milk

摘要: Listeria monocytogenes, a gram-positive bacterium, is of major concern to the food industry. This bacterium pathogenic both humans and animals, particularly susceptible individuals such as pregnant women, newborns, people over 65 years old, immunocompromised patients. L. monocytogenes widely distributed in environment, including soil food. Well-documented outbreaks listeriosis have been associated with consumption contaminated products milk, soft cheese, ready-to-eat meats (10). Because can survive foods under harsh conditions, high acidity low temperature, it very difficult eliminate this pathogen from and/or processing plants. The psychrotrophic nature allows grow at refrigeration temperatures (9, 35); therefore, refrigerated storage no guarantee protection against growth foods. Thus, special precaution should be taken prevent contamination refrigerated/ready-to-eat by microorganism. The ability contaminate milk presents safety public health (18). certain types Mexican-style cheese made raw has linked sporadic cases (6, 20, 23). Physiological studies revealed that was able 4°C (25, 33, 35). However, molecular mechanism its remains unclear. Therefore, there great need for an understanding how grows subsequently pathogen. The availability whole-genome sequence information several food-borne pathogens, paved way global analysis microbial adaptation various environments (1). For example, genomic proteomic approaches were used study behavior lactic bacteria (12, 39). Genes Escherichia coli O157:H7 are involved high-pressure resistance also identified using tools genomics (24). Recently, microarrays responses Bacillus subtilis cold stress, number genes differentially expressed (5, 7). pathogens real matrix generally lacking. The primary goal paper investigate gene expression profile(s) strain F2365 during incubation ultrahigh-temperature-processed (UHT) skim microarray technology. Our objective identify whose patterns altered UHT milk. Such provide baseline data useful adaptations pose risks, dairy products.