A comparative study of three approaches to the routine quantitation of human serum proteins.

摘要: The Laser Nephelometer PDQ™ (Hyland Division, Travenol Laboratories Inc.) and the Abbott Bichromatic Analyser 100 (Abbott Laboratories) were compared with a radial immunodiffusion method. Seventy-eight serum specimens collected during routine blood testing aliquoted quantitated by three procedures described. nephelometric system was used as described Hyland in their instructions accompanying LAS-R Nephelometric test kits. ABA-100 filter unit transmitting at 340 650 nm water bath temperature of 30°C. correlated well RID giving correlation coefficient varying from 0.94 for IgA to 0.79 C3. It not possible quantitate turbidometric method using ABA-100. Results obtained other proteins satisfactory varied 0.88 IgG 0.90 procedure use takes 5 hours technologist time 16-hour incubation period produce results IgG, IgM on nine patient specimens. Using obtain same 20 took two hours. Nephelometry, therefore, appears be alternative comparable precision great saving time.