An enzyme immunoassay for the quantitation of IgG in serum and cerebrospinal fluid.
作者： Jean-Jacques Arseneault
DOI: 10.1016/0009-8981(80)90416-7
关键词: Cerebrospinal fluid sample 、 Antibody 、 Cerebrospinal fluid 、 Coefficient of variation 、 Human serum albumin 、 Chromatography 、 Radial immunodiffusion 、 Immunoassay 、 Chemistry 、 Molecular biology 、 Enzyme
摘要: Abstract An enzyme immunoassay for the determination of IgG in serum and cerebrospinal fluid was devised evaluated to see if it performed clinically acceptable standards. For normal levels between day coefficient variation less than 13%. Using patient material, this new assay compared with radial immunodiffusion; 68 sera there a correlation 0.939 49 specimens, 0.825. The range wider that showed greater sensitivity lowest value determined 1.0 mg/l sample. No linearity aberration or matrix effects were observed. There no appreciable interference, under conditions, from immunoglobulins A M, kappa lambda light chains, human albumin. Recoveries about 100% over whole range. procedure has proven be simple relatively rapid. Assay results are available within 5 h. reagents stable more 6 months.
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