Withania somnifera Suppresses Tumor Growth of Intracranial Allograft of Glioma Cells

摘要: Gliomas are the most frequent type of primary brain tumor in adults. Their highly proliferative nature, complex cellular composition, and ability to escape therapies have confronted investigators for years, hindering advancement toward an effective treatment. Agents that safe can be administered as dietary supplements always remained priority feasible cancer therapy. Withania somnifera (ashwagandha) is essential ingredient Ayurvedic preparations known eliminate cells derived from a variety peripheral tissues. Although our previous studies addressed vitro anti-proliferative differentiation-inducing properties ashwagandha on neuronal cell lines, vivo validating same lacking. While exploring mechanism its action vitro, we observed water extract (ASH-WEX) induced G2/M phase blockade caused activation multiple pro-apoptotic pathways, leading suppression cyclin D1, bcl-xl, p-Akt, reduced expression polysialylated form neural adhesion molecule (PSA-NCAM) well activity matrix metalloproteinases. ASH-WEX intracranial volumes suppressed tumor-promoting proteins p-nuclear factor kappa B (NF-κB), vascular endothelial growth (VEGF), heat shock protein 70 (HSP70), PSA-NCAM, D1 rat model orthotopic glioma allograft. Reduction glial fibrillary acidic (GFAP) upregulation mortalin (NCAM) specifically tumor-bearing tissue further indicated anti-glioma efficacy vivo. Combining this enhanced understanding molecular mechanisms with system offers new opportunities develop therapeutic strategy safe, specific, formulations treating tumors.