Liver cell turnover in rats fed a choline-devoid diet.

摘要: Liver DNA was labeled in a group of weanling Fischer-344 male rats by placing mini-osmotic pumps, loaded with [methyl-3H]thymidine, under the dorsal skin for 14 days. The animals were then placed on either choline-supplemented or choline-devoid diet, and subgroups each diet killed after 1, 2, 4, 8 16 weeks. total specific radioactivities determined all rats. results used to estimate half-life (t1/2) liver cells, fractional rates cell death (Kd) proliferation (Kp). In fed control cells estimated die at an overall Kd 0.16% per day, have t1/2 439.5 days; Kp higher younger than older ranging from 4.82 down 0.93% as length feeding period increased; corresponding t1/2S 14.3 74.6 these rats, more sufficient compensate loss. show that liver-cell represents major consequence necrogenic action is factor responsible highly increased turnover present animals. However, evidence obtained has also primary mitogenic action, beyond those related replacement dead accretion due normal growth liver.