Immunohistochemical analysis of in vivo patterns of TRAF-3 expression, a member of the TNF receptor-associated factor family.

摘要: An immunohistochemical approach was used to explore the in vivo expression of TNF receptor-associated factor 3 (TRAF-3), a putative signaling protein that binds cytosolic domains CD30, CD40, and lymphotoxin-beta receptors. TRAF-3 immunostaining detected many types cells throughout human body. only rarely present thymocytes but found thymic epithelioreticular cells. Lymphocytes bone marrow were also typically immunonegative, whereas myeloid progenitor megakaryocytes often positive. Peripheral blood lymphocytes mostly while granulocytes immunopositive. Monocytes strongly immunostained for TRAF-3, macrophages nodes contained little or no immunoreactivity. Some within germinal centers secondary lymphoid follicles normal reactive immunopositive, as occasional interfollicular T cell regions these organs, most appeared be immunonegative stained weakly. Plasma cells, however, Stimulation PBLs with anti-CD3 Ab induced marked increases steady state levels vitro determined by immunoblotting, TRAF-2 unchanged, implying dynamic regulation expression. The findings establish first time type- differentiation-specific patterns member TRAF family proteins.