RAPD ANALYSIS OF CALLUS REGENERATED AND SEED GROWN PLANTS OF MAIZE (Zea mays L.)

摘要: Genetic and cytogenetic changes are frequently observed in plants regenerated from tissue culture. This phenomenon, termed somaclonal variation, is undesirable genetic transformation processes. DNA-based molecular markers have been used to characterize evaluate variability among individuals. The RAPD technique was variation maize derived culture the inbred line L48 (derived Suwan). Forty seven different decamer oligonucleotide primers generated 221 amplification products, 130 of them being polymorphic. divergence calculated by Nei´s distance based on presence (1) or absence (0) DNA bands analyzed samples. Cluster analyses divided samples into three distinct groups, considering an upper limit 0.38 distances. first group included culture-derived calli , second one plants, seed-derived formed last group. results indicated that segregation still present after six cycles selfing and/or clones same age accumulated types modifications (0.05 distance). These seem be directly correlated with potential for callus regeneration. As increased 13 25 months, regeneration decreased about 28 times. indicate can as a potent easy-to-use tool identify lines less susceptible when cultured vitro, fundamental step plant transformation.