Silencing LINC00511 inhibits cell proliferation, migration, and epithelial–mesenchymal transition via the PTEN–AKT–FOXO1 signaling pathway in lung cancer
作者： Lianyong Jiang , Xiao Xie , Fangbao Ding , Ju Mei , Rui Bi
关键词: Gene knockdown 、 Protein kinase B 、 Cell migration 、 PTEN 、 Biology 、 Cancer 、 Gene silencing 、 Oncogene 、 Cancer research 、 Epithelial–mesenchymal transition
摘要: Lung cancer is the most common cause of cancer-related death worldwide. Long noncoding RNAs (lncRNAs) are longer than 200 nt transcripts and not translated into proteins. Increasing evidence has shown that lncRNAs associated with several biological processes in cancer. However, roles LINC00511 lung progression remain unknown. In present study, we confirmed knockdown significantly inhibited cell proliferation migration A549, SPCA1, H460 cells. Western blot results showed silencing epithelial-mesenchymal transition (EMT), which resulted decreased expression levels ZEB2, N-cadherin, vimentin increased E-cadherin. Additionally, upregulated PTEN mRNA protein expression, FOXO1, inactivated AKT. Furthermore, found reversed inhibition induced by siRNA, markedly reduced p-FOXO1 promoted p-AKT EMT A549 Therefore, these findings revealed functions as an oncogene through PTEN-AKT-FOXO1 signaling pathway cancer, providing a potential target metastasis
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