Isotropic bicelles stabilize the functional form of a small multidrug-resistance pump for NMR structural studies.

摘要: Detergent micelles are commonly used as solubilization agents in biophysical and biochemical studies of membrane proteins, but they do not ideally reproduce the environment often fail to support native protein conformation. Bicelles, which a mixture short- long-chain lipids, have long been suggested more native-like solubilizing agent for study proteins. We tested use isotropic bicelles system solution NMR proteins on small multidrug-resistance (Smr), that has so far resisted unambiguous structural characterization by X-ray crystallography. show can be reconstituted its functional form oligomerization state bicelles. With an assignment strategy makes sequential NOE information obtained from NOESY−TROSY amino-acid specific TROSY-HNCA experiment, 55% backbone HN, N, Cα resonances could assigned, showing isotropic...