Characterization and molecular basis of heterogeneity of the African swine fever virus envelope protein p54.

摘要: It has been reported that the propagation of African swine fever virus (ASFV) in cell culture generates viral subpopulations differing protein p54 (C. Alcaraz, A. Brun, F. Ruiz-Gonzalvo, and J. M. Escribano, Virus Res. 23:173-182, 1992). A recombinant bacteriophage expressing a 328-bp fragment gene was selected lambda phage expression library ASFV genomic fragments by immunoscreening with antibodies against protein. The sequence this allowed location EcoRI E genome. Nucleotide obtained from revealed an open reading frame encoding 183 amino acids calculated molecular weight 19,861. This contains transmembrane domain Gly-Gly-X motif, recognition for processing several structural proteins. In addition, two direct tandem repetitions were also found within frame. Further characterization transcription product is translated late mRNA incorporated to external membrane particle. comparison nucleotide carried virulent strains (E70 E75) Ba71V showed 100% similarity. However, when genes clones generated passage coding proteins different electrophoretic mobility sequenced, they changes number copies 12-nucleotide repeat. These produce alterations acid Pro-Ala-Ala-Ala present p54, resulting stepwise modifications duplications deletions repeat array region constitute novel mechanism genetic diversification ASFV.