Repair and mutagenic potential of oxaluric acid, a major product of singlet oxygen-mediated oxidation of 8-oxo-7,8-dihydroguanine.

摘要: Oxidative reactions within DNA commonly result in base modifications. Among the four bases, guanine is most susceptible to various oxidants, and its related oxidized form, 8-oxo-7,8-dihydroguanine, has been extensively studied terms of repair mutagenicity. However, 8-oxo-7,8-dihydroguanine readily subjected further oxidation, this become a point interest. We recently found that singlet oxygen oxidation led predominant formation oxaluric acid as final product. report herein on biological features dealing with vitro synthesis removal from by enzymes. Nucleotide insertion opposite acid, catalyzed Kf exo- Taq indicates, induces G T C transversions. On other hand, represents block when performed pol β. Interestingly, experiments carried out formamidopyrimidine N-glycosylase (Fpg) e...