Temporal Dynamic Changes of Phenotypic Expression of Peripheral CD4 Cells during Environmental Allergen Challenge in an Experimental Model of Canine Atopic Dermatitis: A Pilot Study
作者： Andrew SIMPSON , Sadatoshi MAEDA , Rosanna MARSELLA
DOI: 10.1292/JVMS.71.1177
关键词: Allergy 、 Peripheral blood mononuclear cell 、 Immunology 、 IL-2 receptor 、 Chemokine 、 Medicine 、 Sensitization 、 Atopic dermatitis 、 Allergen 、 Environmental exposure
摘要: The immunopathology behind atopic dermatitis (AD) involves a myriad of inflammatory cells and their mediators. Thymus activation-regulated chemokine (TARC) plays significant role in the phase by recruiting CCR4+ TH2 cells. In addition, CD25+ activated T further propagate allergic response after sensitization producing cytokines. purpose this pilot study was to evaluate how exposure common allergen (house dust mite, HDM) would affect proportions circulating CD4+CCR4+ CD4+CD25+activated an experimental model AD using high-IgE Beagles. model, previously sensitized Beagles develop lesions pruritus upon challenge consisting 3-day environmental exposure, 3 hours/day. Blood samples were obtained before, during, end (days 0, 2, 4, 17). Clinical signs evaluated scored at same time points. Peripheral blood mononuclear (PBMCs) isolated used for flow cytometry identify CD4+cells positive either CCR4 or CD25 receptors. Both CD4+cell types (CD25+ CCR4+) peaked day 17, when clinical had resolved. It is proposed that increase CD4+CD25+cells most likely demonstrates status individuals challenge. Understanding these two cell could prompt additional research concerning therapeutic drugs AD.
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