Nontyphoid Salmonella gastroenteritis in pediatric patients from urban areas in the city of Mérida, Venezuela

摘要: Background: Nontyphoid Salmonella (NTS) infections are a frequent cause of self-limited diarrhoeal illness in healthy children that do not usually require antibiotic treatment. This study was conducted by analyzing the phenotypic and genotypic traits NTS strains from pediatric patients with acute gastroenteritis living urban areas city Merida, Venezuela. Methodology: Thirty-seven (18 S. Enteritidis; 14 Typhimurium; 2 Java; Saintpaul; 1 Infantis) were isolated 243 stool specimens. These biochemically identified serotyped. Antimicrobial susceptibility determined disc-diffusion assay. Genetic characterization included plasmid profiling, PCR detection spv region inv genes, IS200 typing. Results: Thirty (81.0%) isolates resistant to antimicrobial tested. Of these strains, 17 (56.7%) at least one antibiotic. Five resistance patterns observed, which most frequently found single type (tetracycline, streptomycin or ampicillin). All Typhimurium harbored plasmids, but only three large plasmids (60, 72 84 kb) yielded amplicons spvR specific primers. serotypes showed presence an region. Eight distinct profiles could be detected among 37 studied. Conclusions: Predominant Enteritidis serotypes, as well Java, Saintpaul Infantis, circulating Venezuela. Most susceptible first-line antibiotics active monitoring for is necessary. IS 200 typing, applied association conventional methods, allowed all suggested different infection sources.