Typing of Salmonella Typhi strains isolated from Egypt by RAPD PCR.

摘要: PCR-based fingerprinting using random amplified polymorphic DNA (RAPD) has been used widely for genome identification. In this study, 13 Salmonella Typhi strains were isolated from typhoid patients Aswan, Cairo, Fayoum, and Monofya Governorates of Egypt. The isolates, along with three reference strains, i.e., O901, H901, Ty2 subjected to whole typing by RAPD PCR. Three RAPD-PCR 10-mer primers generated a total 85 bands (81 bands), 12 distinct PCR profiles, proved be useful discriminating the isolates studied. Interestingly, B1 C1 profile found only in Cairo Monofya, respectively; some types appeared certain By combining profiles obtained primer trio an excellent discrimination index (D) 0.942 was reached. Pairwise comparisons Jaccard’s similarity coefficients calculated among identified major clusters; O901 branch H901 sub-branches. Principal component analysis adequately resolved each these clusters. principal components accounted about 72% variation, first two accounting 62% variance genotypes Biclustering improved display groups amplicons (markers) that cluster similarly across genomes could delineate features pertaining structure. conclusion, provided fast method high potentials surveillance epidemiological investigations infections.