ERIC PCR and RAPD based fingerprinting of Salmonella Typhi strains isolated over a period of two decades

摘要: Abstract Salmonella enterica serotype Typhi strains ( n  = 113) were isolated from typhoid patients over a period of 2 decades, i.e. 1987–2006. RAPD and ERIC PCR methods used for random whole genome typing these strains. was found to be very efficient with the discriminatory index (DI) 0.9821 100% reproducibility. satisfactory in discriminating (DI = 0.8978) but poor reproducibility (40%). However, composite genotypic analysis still better DI 0.9981 inherent due RAPD. Two major clones observed circulating community few unrelated too. The dendrogram constructed based on banding pattern by involving 89 revealed 71 patterns, indicating that bacterium is capable rapid changes variations. Thus, spectrum biological manifestations human infection S . may related its capacity genetic diversity underlined highly plastic hypermutable genome.