作者: José L. Romero , María J. Grande Burgos , Rubén Pérez-Pulido , Antonio Gálvez , Rosario Lucas
关键词: Biocide 、 Bacteria 、 Microbiology 、 Pseudomonas 、 Antimicrobial 、 Antibiotics 、 Acinetobacter 、 Biology 、 Aeromonas 、 Antibiotic resistance
摘要: Multi-drug resistant bacteria (particularly those producing extended-spectrum β-lactamases) have become a major health concern. The continued exposure to antibiotics, biocides, chemical preservatives and metals in different settings such as the food chain or environment may result development of multiple resistance co-resistance. aim present study was determine resistances (biocides, preservatives, phenolic compounds metals) bacterial isolates from seafoods. A 75.86% 87 studied were at least one antibiotic biocide, 6.90% multiply three biocides antibiotics. Significant (P < 0.05) moderate strong positive correlations detected between tolerances antibiotics with other antimicrobials. sub-set 30 selected according antimicrobial profile type identified by 16S rDNA sequencing tested for copper zinc tolerance. Then, genetic determinants biocide metal tolerance investigated. Pseudomonas (63.33%), Acinetobacter (13.33%), Aeromonas Shewanella, Proteus Listeria (one isolate each). Antibiotic included sul1 (43.33% isolates), sul2 (6.66%), blaTEM (16.66%), blaCTX-M blaPSE (10.00%), blaIMP (3.33%), blaNDM-1 floR aadA1 (20.0%) aac(6’)-Ib (16.66%). only determinant among qacEΔ1 (10.00%). 23.30 able grow on media containing 32 mM sulfate, 46.60% 8 chloride. genes pcoA/copA, pcoR chrB 36.66%, 6.66% 13.33% isolates, respectively. Twelve both genes, including carbapenemase gene pcoA/copA. These results suggest that could co-select also highlight potential seafoods be involved transmission genes.