An efficient regeneration system and steviol glycoside analysis of Stevia rebaudiana Bertoni, a source of natural high-intensity sweetener

摘要: Stevia is a natural, zero-calorie, intensively sweet extracted from the leaves of rebaudiana. Its taste derives group compounds known as steviol glycosides. In this study, an efficient micropropagation protocol for S. rebaudiana was developed possible commercial implementation using Murashige and Skoog (MS) basal medium without plant growth regulators most process. Direct shoot formation achieved after cultivation nodal segments on MS with or (benzyl amino purine [BAP], kinetin [KIN]) at various concentrations (0.1, 0.5, 1.0, 2.0 mg L−1). Although all treatments produced two shoots per explants 3 wk culture, high KIN BAP (1.0 L−1) induced more callus formation. Rooting containing 0.25 L−1 indole-3-acetic acid (IAA), which 8.1 roots cultivation. After acclimatization regenerants in portable greenhouse wk, seed-derived seedlings were transferred to field conditions 16 wk. Steviol glycoside contents (% leaf dry weight) did not differ between collected plants. Rebaudioside A content ranged 4.7 5.0% (w/w), while stevioside 6.4 6.9% (w/w). There no significant difference sampling periods (late vegetative flowering stages) plants grown field. cost-effective vitro regeneration established that enables efficient, large-scale production