CXXC Finger Protein 1 Is Required for Normal Proliferation and Differentiation of The PLB-985 Myeloid Cell Line
作者： Suzanne R.L. Young , David G. Skalnik
关键词: Transfection 、 Biology 、 DNA methyltransferase 、 DNA methylation 、 Molecular biology 、 Histone methyltransferase complex 、 Clone (cell biology) 、 CpG site 、 DNA 、 Small hairpin RNA
摘要: CXXC finger protein 1 (CFP1) binds to unmethylated CpG motifs in DNA, is a component of the mammalian Set1 histone methyltransferase complex, and essential for zebrafish hematopoiesis. Transfection human PLB-985 myeloid cell line with short hairpin RNA directed against transcript encoding CFP1 results 80% fewer colonies compared vector control, suggesting that required survival cells. One clone, CFP1–AS1, exhibits 70% decrease levels slower doubling time due an increase proportion cells G1 G2 S phase. CFP1–AS1 exhibit 40% reduction DNA but contain normal global genomic cytosine methylation. The clone suffers from defect granulocytic differentiation, as approximately half fail obtain terminally differentiated nuclear architecture generate respiratory burst. Similar were obtained upon induc...
liebertonline.com参考文章(58)
Q Shen, S Chada, C Whitney, PE Newburger, Regulation of the human cellular glutathione peroxidase gene during in vitro myeloid and monocytic differentiation. Blood. ,vol. 84, pp. 3902- 3908 ,(1994) , 10.1182/BLOOD.V84.11.3902.BLOODJOURNAL84113902
KA Tucker, MB Lilly, L Jr Heck, TA Rado, Characterization of a new human diploid myeloid leukemia cell line (PLB-985) with granulocytic and monocytic differentiating capacity. Blood. ,vol. 70, pp. 372- 378 ,(1987) , 10.1182/BLOOD.V70.2.372.372
M. Szyf, V. Bozovic, G. Tanigawa, Growth regulation of mouse DNA methyltransferase gene expression. Journal of Biological Chemistry. ,vol. 266, pp. 10027- 10030 ,(1991) , 10.1016/S0021-9258(18)99179-9
Allen Shearn, Amy L. Adamson, Molecular Genetic Analysis of Drosophila Ash2, a Member of the Trithorax Group Required for Imaginal Disc Pattern Formation Genetics. ,vol. 144, pp. 621- 633 ,(1996) , 10.1093/GENETICS/144.2.621
Binghui Li, Feng-Chun Yang, D. Wade Clapp, Kristin T. Chun, Enforced expression of CUL-4A interferes with granulocytic differentiation and exit from the cell cycle Blood. ,vol. 101, pp. 1769- 1776 ,(2003) , 10.1182/BLOOD-2002-05-1517
Qiang Fu, Robert L. Jilka, Stavros C. Manolagas, Charles A. O'Brien, Parathyroid Hormone Stimulates Receptor Activator of NFκB Ligand and Inhibits Osteoprotegerin Expression via Protein Kinase A Activation of cAMP-response Element-binding Protein Journal of Biological Chemistry. ,vol. 277, pp. 48868- 48875 ,(2002) , 10.1074/JBC.M208494200
STEVEN J. COLLINS, ROBERT C. GALLO, ROBERT E. GALLAGHER, Continuous growth and differentiation of human myeloid leukaemic cells in suspension culture. Nature. ,vol. 270, pp. 347- 349 ,(1977) , 10.1038/270347A0
Diana L. Carlone, Jeong-Heon Lee, Suzanne R. L. Young, Erika Dobrota, Jill Sergesketter Butler, Joseph Ruiz, David G. Skalnik, Reduced genomic cytosine methylation and defective cellular differentiation in embryonic stem cells lacking CpG binding protein. Molecular and Cellular Biology. ,vol. 25, pp. 4881- 4891 ,(2005) , 10.1128/MCB.25.12.4881-4891.2005
Y. Gu, T. Nakamura, H. Alder, R. Prasad, O. Canaani, G. Cimino, C.M. Croce, E. Canaani, The t(4;11) chromosome translocation of human acute leukemias fuses the ALL-1 gene, related to Drosophila trithorax, to the AF-4 gene Cell. ,vol. 71, pp. 701- 708 ,(1992) , 10.1016/0092-8674(92)90603-A
Suzanne R. L. Young, Christen Mumaw, James A. Marrs, David G. Skalnik, Antisense targeting of CXXC finger protein 1 inhibits genomic cytosine methylation and primitive hematopoiesis in zebrafish. Journal of Biological Chemistry. ,vol. 281, pp. 37034- 37044 ,(2006) , 10.1074/JBC.M604546200