Chronic Lymphocytic Leukemic B Cells But Not Normal B Cells Are Rescued From Apoptosis by Contact With Normal Bone Marrow Stromal Cells
作者： L. Lagneaux , A. Delforge , D. Bron , C. De Bruyn , P. Stryckmans
关键词: CD40 、 Medicine 、 Bone marrow 、 Cytokine 、 Cancer research 、 Pathology 、 Cell–cell interaction 、 Lymph node stromal cell 、 Apoptosis 、 Leukemia 、 Stromal cell
摘要: The leukemic B lymphocytes from chronic lymphocytic (CLL) patients have a long survival in vivo, although ex vivo they rapidly die by apoptosis. To further investigate the mechanism of this, we studied influence bone marrow stromal cells normal subjects on apoptosis B-CLL and umbilical cord blood (UCB) lymphocytes. After 48 hours incubation medium alone, showed, respectively, 22 +/- 3% 31 5% Cocultures with reduced percentage undergoing (8 2%, P < . 0005) prevented loss bcl-2 protein expression. In contrast, slightly increased B-cell (37 6%). Direct contact between was found to be essential for inhibition cell apoptosis; indeed, separation microporous membrane spontaneous apoptosis, comparable results were obtained conditioned medium. difference behavior observed plated can explained fact that only few adhere comparison cells. adhesion is mediated beta1 beta2 integrins acting simultaneously. Contact seems play major role accumulation marrow.
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PC Kierney, K Dorshkind, B lymphocyte precursors and myeloid progenitors survive in diffusion chamber cultures but B cell differentiation requires close association with stromal cells. Blood. ,vol. 70, pp. 1418- 1424 ,(1987) , 10.1182/BLOOD.V70.5.1418.1418
K Bradstock, V Makrynikola, D Gottlieb, A Bianchi, R Filshie, Long-term survival and proliferation of precursor-B acute lymphoblastic leukemia cells on human bone marrow stroma Leukemia. ,vol. 10, pp. 813- 820 ,(1996)
I Dubé, S Kamel-Reid, B D Lichty, J L Hummel, M Reis, Engraftment of human chronic lymphocytic leukemia cells in SCID mice: in vivo and in vitro studies Leukemia. ,vol. 10, pp. 1370- 1376 ,(1996)
T Mainou-Fowler, VA Craig, JA Copplestone, MD Hamon, AG Prentice, Interleukin-5 (IL-5) increases spontaneous apoptosis of B-cell chronic lymphocytic leukemia cells in vitro independently of bcl-2 expression and is inhibited by IL-4. Blood. ,vol. 84, pp. 2297- 2304 ,(1994) , 10.1182/BLOOD.V84.7.2297.2297
AM Vincent, JC Cawley, J Burthem, Integrin function in chronic lymphocytic leukemia. Blood. ,vol. 87, pp. 4780- 4788 ,(1996) , 10.1182/BLOOD.V87.11.4780.BLOODJOURNAL87114780
David J McConkey, Joya Chandra, Michael Keating, Timothy J McDonnell, William K Plunkett, Susan Wright, John C. Reed, Apoptosis sensitivity in chronic lymphocytic leukemia is determined by endogenous endonuclease content and relative expression of BCL-2 and BAX. Journal of Immunology. ,vol. 156, pp. 2624- 2630 ,(1996)
L Lagneaux, A Delforge, C Dorval, D Bron, P Stryckmans, Excessive production of transforming growth factor-beta by bone marrow stromal cells in B-cell chronic lymphocytic leukemia inhibits growth of hematopoietic precursors and interleukin-6 production Blood. ,vol. 82, pp. 2379- 2385 ,(1993) , 10.1182/BLOOD.V82.8.2379.2379
A Manabe, KG Murti, E Coustan-Smith, M Kumagai, FG Behm, SC Raimondi, D Campana, Adhesion-Dependent Survival of Normal and Leukemic Human B Lymphoblasts on Bone Marrow Stromal Cells Blood. ,vol. 83, pp. 758- 766 ,(1994) , 10.1182/BLOOD.V83.3.758.758
M Forveille, A Durandy, L Thuillier, A Fischer, Phenotypic and functional characteristics of human newborns' B lymphocytes. Journal of Immunology. ,vol. 144, pp. 60- 65 ,(1990)
J. L. Liesveld, Camille Abboud, D. H. Ryan, B. L. Nuccie, Maturation-dependent adhesion of human B cell precursors to the bone marrow microenvironment. Journal of Immunology. ,vol. 145, pp. 477- 484 ,(1990)