A novel human myosin alkali light chain is developmentally regulated. Expression in fetal cardiac and skeletal muscle and in adult atria.
作者： Hans-Henning ARNOLD , Peter LOHSE , Ulla SEIDEL , Eva BOBER
DOI: 10.1111/J.1432-1033.1988.TB14428.X
关键词: MYH7 、 Gene expression 、 Skeletal muscle 、 Recombinant DNA 、 Molecular biology 、 Gene isoform 、 Complementary DNA 、 Myosin light-chain kinase 、 Myosin 、 Biology
摘要: We have isolated cDNA recombinant phages encoding the embryonic isoform of myosin alkali light chain (MLC1emb) from a human fetal skeletal muscle library. The clones were detected by their weak cross-hybridization to MLC1F and MLC3F clone. Nucleotide sequence analysis complete (GT14) revealed an open reading frame for 197 amino acids. derived protein constitutes first structural information on this any organism. Remarkable similarities other MLC polypeptides, particularly those slow-muscle type, are evident. Under conditions high stringency, GT14 clone hybridized abundant mRNA species in ventricular adult atrial muscle, whereas only very weakly hybridizing component was detected. These mRNAs indistinguishable size thermal stability hybrids formed with DNA insert GT14. therefore conclude that identical is expressed these tissues, presumably transcribed same gene. According its pattern expression, novel described here designated as “embryonic chain” (MLC1emb/A) reference developmental stage-specific tissue-specific appearance ventricle atrium.
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